1、固氮螺菌培养基聽(ATCC聽838)聽
K2HPO4聽聽聽聽聽聽聽聽聽聽0.1g聽聽聽聽聽KH2PO4聽聽聽聽聽聽聽聽0.4g聽
MgSO4.7H2O聽聽聽聽聽聽0.2g聽聽聽聽聽NaCl聽聽聽聽聽聽聽聽聽聽0.1g聽
CaCl2聽聽聽聽聽聽聽聽聽聽聽0.02g聽聽聽聽FeCl3聽聽聽聽聽聽聽聽聽0.01g聽
NaMoO4.2H2O聽聽聽聽聽0.002g聽聽聽苹果酸钠聽聽聽聽聽聽5.0g聽
酵母膏聽聽聽聽聽聽聽聽聽聽0.05g聽聽聽聽蒸馏水聽聽聽聽聽聽聽聽1000ml聽
pH聽7.2-7.4聽
2、BG-11聽
NaNO3聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽1.5g聽聽聽聽聽聽聽聽聽K2HPO4聽聽聽聽聽聽聽聽聽聽聽0.04g聽
MgSO4.7H2O聽聽聽聽聽聽聽聽聽聽0.075g聽聽聽聽聽聽聽CaCl2.7H2O聽聽聽聽聽聽聽0.036g聽
Na2CO3聽聽聽聽聽聽聽聽聽聽聽聽聽聽0.02g聽聽聽聽聽聽聽聽柠檬酸聽聽聽聽聽聽聽聽聽聽聽0.006g聽
柠檬酸铁聽聽聽聽聽聽聽聽聽聽聽聽0.006g聽聽聽聽聽聽聽微量元素溶液聽A5聽聽1ml聽
氨苄青霉素聽(终浓度)聽50μg/ml聽聽聽聽聽聽蒸馏水聽聽聽聽聽聽聽聽聽聽聽1000ml聽
琼脂聽20.0g聽
*聽微量元素溶液聽A5：
H3BO4聽聽聽聽聽聽聽聽2.86g聽聽聽聽MnCl2.4H2O聽聽聽聽聽1.81g聽
ZnSO4聽聽聽聽聽聽聽聽0.222g聽聽聽Na2MoO4聽聽聽聽聽聽聽聽0.39g聽
CuSO4.5H2O聽聽聽0.079g聽聽聽Co(NO3)2.6H2O聽聽49.4g聽
3、(Marine聽Agar)聽
蛋白胨聽聽聽聽聽聽聽5.0g聽聽聽聽聽聽酵母膏聽聽聽聽1.0g聽
柠檬酸铁聽聽聽聽聽0.1g聽聽聽聽聽聽NaCl聽聽聽聽聽聽19.45g聽
MgSO4.7H2O聽聽聽5.9g聽聽聽聽聽聽KCl聽聽聽聽聽聽聽0.55g聽
Na2SO4聽聽聽聽聽聽聽3.24g聽聽聽聽聽CaCl2聽聽聽聽聽1.8g聽
NaHCO3聽聽聽聽聽聽聽0.16g聽聽聽聽聽KI聽聽聽聽聽聽聽聽0.08g聽
微量元素聽聽聽聽聽10ml聽聽聽聽聽聽pH聽7.0-7.6聽
*微量元素溶液：聽
氯化锶聽聽聽0.034g聽聽聽聽聽聽聽聽硅酸钠聽聽0.004g聽
蒸馏水聽聽聽1000ml聽聽聽聽聽聽聽聽H3BO4聽聽聽0.022g聽
NaCl聽聽聽聽聽0.0024g聽聽聽聽聽聽聽NaNO3聽聽聽0.0016g聽
NaHPO4聽聽聽0.008g聽聽聽聽聽聽聽聽蒸馏水聽聽1000ml聽
4、（Yeast聽Starch聽Agar）
酵母膏聽2.0g聽聽聽聽聽聽可溶性淀粉聽10.0g聽
琼脂聽聽聽20.0g聽聽聽聽聽蒸馏水聽聽聽聽聽1000ml聽
pH聽7.2-7.4聽
5、GYMC聽
葡萄糖聽4.0g聽聽酵母膏聽2.0g聽
麦芽汁聽20.0g聽琼脂聽聽聽20.0g聽
CaCO3聽聽1.5g聽聽蒸馏水聽1000ml聽
pH聽6.8-7.0聽
6、聽LCSB聽
乳糖聽聽聽聽聽聽聽聽聽聽15.0g聽聽聽聽玉米浆聽聽聽聽聽聽聽5.0g聽
蛋白胨聽聽聽聽聽聽聽聽5.0g聽聽聽聽聽NaCl聽聽聽聽聽聽聽聽聽4.0g聽
MgSO4.7H2O聽聽聽聽0.5g聽聽聽聽聽KH2PO4聽聽聽聽聽聽聽0.6g聽
FeCl3.6H2O聽聽聽聽0.005g聽聽聽CuSO4.5H2O聽聽聽0.002g聽
琼脂聽聽聽聽聽聽聽聽聽聽20.0g聽聽聽聽聽蒸馏水聽聽聽聽聽聽1000ml聽
相对湿度聽65%聽聽聽聽聽聽聽聽聽聽聽pH聽4.8聽
7、Tep尿液(Uric聽Acid)琼脂(ATCC聽894)聽
Na2HPO4.12H2O聽聽聽聽9.0g聽聽聽聽KH2PO4聽聽聽聽聽聽聽聽聽聽聽1.5g聽
MgSO4.7H2O聽聽聽聽聽聽聽0.2g聽聽聽聽柠檬酸铁铵(绿色)聽1.2g聽
CaCl2聽聽聽聽聽聽聽聽聽聽聽聽20.0mg聽MnCl2.4H2O聽聽聽聽聽聽聽聽1.0mg聽
Trypticcase聽Soy聽Broth聽(BBL聽11768)聽聽聽聽聽聽聽聽聽3.0g聽
尿素聽聽聽聽聽聽聽聽聽聽聽聽聽聽4.0g聽聽聽聽聽琼脂聽聽聽聽聽聽聽聽聽聽聽20.0聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽
蒸馏水聽聽聽聽聽聽聽聽聽聽聽聽1000ml聽聽聽pH聽7.2聽
8、增强梭菌生长的培养基聽(ATCC聽1053)聽
胰蛋白胨聽聽聽聽聽聽聽聽聽10.0g聽聽聽聽聽牛肉膏聽聽聽聽聽10.0g聽
酵母膏聽聽聽聽聽聽聽聽聽聽聽3.0g聽聽聽聽聽聽葡萄糖聽聽聽聽聽聽5.0g聽
NaCl聽聽聽聽聽聽聽聽聽聽聽聽聽5.0g聽聽聽聽聽聽可溶性淀粉聽聽1.0g聽
半胱氨酸盐酸盐聽聽聽0.5g聽聽聽聽聽聽聽醋酸钠聽聽聽聽聽3.0g聽
琼脂聽聽聽聽聽聽聽聽聽聽聽聽聽15.0g聽聽聽聽聽聽蒸馏水聽聽聽聽聽1000ml聽
pH聽6.6-7.0聽
9、豆饼粗粉琼脂培养基聽（AS聽20）聽
10%豆饼粗粉浸液聽聽聽1000g聽葡萄糖聽聽聽10.0g聽
NaCl聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽2.5g聽聽聽琼脂聽聽聽15.0g聽
10、北京棒状杆菌噬菌体培养基聽（AS聽48）聽
（1）牛肉膏聽3.0g聽聽聽聽聽蛋白胨聽5.0g聽
聽聽聽聽聽NaCl聽聽聽5.0g聽聽聽聽聽葡萄糖聽10.0g聽
聽聽聽聽聽蒸馏水聽1000ml聽聽聽pH聽7.0聽
（2）葡萄糖聽聽聽聽聽聽聽20.0g聽聽聽聽聽聽聽玉米浆聽聽聽聽聽聽聽聽聽8.0g聽
聽聽聽聽聽K3PO4.3H2O聽聽聽1.0g聽聽聽聽聽聽聽聽MgSO4.7H2O聽聽聽聽聽0.4g聽
聽聽聽聽聽尿素（Urea）聽5.0g聽聽聽聽聽聽聽聽Fe+,Mn+分别为聽聽2聽ppm聽
聽聽聽聽聽蒸馏水聽聽聽聽聽聽聽1000ml聽聽聽聽聽聽pH聽7.0聽
聽聽聽
聽聽聽

聽

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